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Promega E. coli S30 T7 High-Yield Protein Expression System

The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.

Supplier:  Promega L1110

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Catalog No. PRL1110

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Includes: T7 S30 Extract, an optimized S30 Premix Plus provides all other required components, including amino acids, rNTPs, tRNAs, an ATP-regenerating system, IPTG and appropriate salts to express high levels of recombinant proteins. Also includes S30 T7 Control DNA, a control DNA template.



  • Simplified Transcription/Translation of DNA Cloned in Plasmid or Lambda Vectors with a T7 Promoter
  • Express up to 500µg/mL of protein in one hour
  • Flexible detection of expressed proteins by Coomassie(R) staining or fluorescence or biotinylated labels


1 hr.
S30 T7 High Yield Protein expression system
24 reactions
Protein-Protein interactions, Protein-DNA interactions, Enzyme activity, Antibody production
500 μg/mL
24 Reactions


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U.S. Patents 5,292,658, and 5,418,155; Canadian Patent 2,105,984 and related patents.