Promega E. coli S30 T7 High-Yield Protein Expression System

Description

Includes

T7 S30 Extract, an optimized S30 Premix Plus provides all other required components, including amino acids, rNTPs, tRNAs, an ATP-regenerating system, IPTG and appropriate salts to express high levels of recombinant proteins. Also includes S30 T7 Control DNA, a control DNA template.

The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.

• Simplified Transcription/Translation of DNA Cloned in Plasmid or Lambda Vectors with a T7 Promoter
• Express up to 500μg/mL of protein in one hour
• Flexible detection of expressed proteins by Coomassie(R) staining or fluorescence or biotinylated labels

Specifications

• Test Time: 1 hr.
• Yield: 500 μg/mL
• Type: E. coli Protein Expression System
• Sufficient For: 24 Reactions
• Quantity: 24 reactions
• Content And Storage: <-65°C
• For Use With (Application): Protein-Protein interactions, Protein-DNA interactions, Enzyme activity, Antibody production

U.S. Patents 5,292,658, and 5,418,155; Canadian Patent 2,105,984 and related patents.