Promega M-MLV Reverse Transcriptase, RNase H Minus

Catalog No. PRM5301

Supplier: Promega M5301

Description

Deletion mutation of M-mLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.

• Helpful for Preparing Long cDNAs and Libraries with a High Percentage of Full-Length cDNA
• Supplied at a concentration of 100-200u/μL

Specifications

• Concentration: 100 to 200 U/μL
• Color: Colorless
• Components: 5X Reaction Buffer: 250mM Tris HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT
• Purity: >90%
• pH: 7.5
• For Use With (Application): First-strand cDNA synthesis, primer extension
• Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet™ P-40, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 10,000 U
• Product Type: M-MLV Reverse Transcriptase, RNase (H-)
• Inhibitors: Recombinant RNasin™ Ribonuclease
• Form: Liquid