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Promega ONE-Glo™ Luciferase Assay System

The ONE-Glo™ Luciferase Assay System provides a sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideal for high- and ultrahigh-throughput applications.

Manufacturer:  Promega E6130

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Catalog No. PRE6130

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Includes: ONE-Glo Luciferase Assay Buffer, ONE-Glo Luciferase Assay Substrate



  • Robust Performance for High- and Ultrahigh-Throughput Reporter Assays
  • Less sensitive to mixing and dispensing conditions, enhancing reproducibility
  • Ideal for use in high-density (384- and 1536-well) microplates
  • Reduced odor, and convenient storage options


1000 mL
1 x 1000mL ONE-Glo™ Luciferase Assay Buffer, 1 x 1 vial ONE-Glo™ Luciferase assay substrate
Detection of firefly luciferase reporter gene expression in mammalian cells
384- and 1536-well plates
Safety and Handling

Safety and Handling

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Patent pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.
Certain applications of this product may require licenses from others.