Promega pFC30A His6HaloTag™ T7 Flexi Vector for E. coli and Cell-Free Protein Expression

Description

These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.

• HaloTag(R)-Polyhistidine Dual Tag Bacterial Expression Vectors
• Express proteins in E. coli T7 cell-free expression systems
• Couple the protein solubility and labeling benefits of HaloTag(R) technology with the reusability and the throughput of Ni-affinity technology
• Select N- or C-terminal tags and either Ampicillin or Kanamycin resistance

Specifications

• Kit Contents: C-terminal His6-HaloTag™ dual tag
• Type: pFC30A His-6HaloTag T7 Flexi Vector
• Sufficient For: E. coli and T7 cell-free Protein expression systems
• Quantity: 20 μg
• Content And Storage: -30°C to -10°C
• For Use With (Application): Bacterial Protein expression and purification, cell-free Protein expression and purification