Promega pGEM™-T and pGEM™-T Easy Vector Systems

Description

Includes

Vector, Control insert DNA, T4 DNA ligase, Ligation buffer, High efficiency competent cells (Mfr. Nos. A3610 and A1380 only)

Convenient systems for the routine subcloning of PCR products

The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid.

• 2X Rapid Ligation Buffer allows reactions to be completed in one hour at room temperature Blue/white screening can be used to directly identify recombinant clones, as T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase, permitting insertional inactivation of the α-peptide
• f1 Origin of Replication allows the preparation of single-stranded DNA

pGEM-T Vector Systems

• Multiple cloning site is flanked by recognition sites for the restriction enzyme BstZ I Single-enzyme digestion allows release of the insert
Double digestion may also be used to release the insert from the vector

pGEM-T Easy Vector Systems

• Recognition sites for BstZ I, EcoR I, and Not I flank the insertion site Several options are available for removal of the desired insert DNA with a single restriction digestion

Specifications

• Promoter: SP6, T7
• Restriction Site: BstZI, NotI and EcoRI
• Content And Storage: -30°C to -10°C
• Quantity: 20 Reactions
• Vector: pGEM-T easy vector I
• For Use With (Application): For the routine subcloning of PCR products