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Promega pGEM™ Vectors

Enable blue/white screening and contain both the SP6 and T7 RNA polymerase promoters

Manufacturer:  Promega P2261

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Catalog No. PR-P2261

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Includes: Vector, 20μg; Bacterial Strain JM109, 500μL



  • Standard Cloning Vectors That Can Produce ssDNA and In vitro Transcribe Sense and Antisense RNA
  • Blue/white screening for easy identification of recombinant clones
  • In vitro transcription using the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • The difference between + and - vectors is orientation of the f1 origin


Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII
pGEM-3Zf(-) vector
Sp6 and T7
20 μg
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