Promega pGL4 Luciferase Reporter Vectors

Description

Available in numerous configurations for enhanced usability and convenience

• Reporter Vectors that Lack a Promoter and Offer Different Reporter Protein Stabilities
• Designed for cloning a putative promoter element for investigation of gene transcription control regions
• Available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP

• For research use only. This product and/or its use is subject to one or more of the following Promega patents: U.S. Pat. Appln. Ser. Nos. 09/645,706; 10/664,341; 10/943,508; U.S. Patents 5,670,356; 6,387,675 and 6,552,179, Australian Patent 698424 and various corresponding patent applications and issued patents. The terms of the limited license conveyed with the purchase of this product are as follows: Researchers may use this product in their own research and they may transfer derivatives to others for such research use provided that at the time of transfer a copy of this label license is given to the recipients and the recipients agree to be bound by the conditions of this label license. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that Researchers may: (1) clone heterologous DNA sequences at either or both ends of said luciferase gene so as to create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus when compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in furtherance of splicing research predicated on the inactivation or reconstitution of the luminescent activity of the encoded luciferase. In addition, Researchers must do one of the following: (1) use luminescent assay reagents purchased from Promega Corporation for all determinations of luminescence activity resulting from the research use of this product and its derivatives; or (2) contact Promega to obtain a license for the use of the product and its derivatives. No other use or transfer of this product or its derivatives is authorized without the express written consent of Promega including, without limitation, Commercial Use. Commercial Use means any and all uses of this product and derivatives by a party for monetary or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to such Commercial Use, or any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. If the purchaser is not willing to accept the conditions of this limited use statement, Promega is willing to accept the return of the unopened product and provide the purchaser with a full refund. However, in the event the product is opened, then the purchaser agrees to be bound by the conditions of this limited use statement.
• Mfr. No. C9351, E1310, E1320, E1370, E6651, E6661, E6671, E6681, E6691, E6701, E6711, E6721, E6731, E6741, E6751, E6761, E6771, E8411, E8421, E8431, E8441, E8451, E8461, E8471, E8481, E8491: The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
• Mfr. No. E6881, E6891, E6901, E6911, E6921, E6931, E6941, E6951, E6961, E6971, E6981, E6991, E7501, E7511, E7521: Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patents 2,105,984 and related patents.
• Mfr. No. E8481: The NFAT response element and its use are licensed under one or more of the following patents: U.S. Patents 5,837,840; 6,197,925; 5,989,810; 6,096,515; 6,388,052; 6,150,099; 6,171,781; 6,352,830; 6,312,899 and 6,875,571.
• Mfr. No. E1310, E1320: The use of this product and derivatives thereof is strictly limited to that of a life sciences research reagent. All other use is strictly prohibited, including but not limited to any diagnostic, therapeutic, or commercial use. The use of this product for in vivo bioluminescent imaging may be covered by one or more patents controlled by Caliper Life Sciences, Inc. Those patents include U.S. Patents 5,650,135; 6,217,847; 6,923,951; 6,908,605; 6,890,515; and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Caliper Life Sciences, Inc., 68 Elm St., Hopkinton, MA 01748 for information regarding a license to practice under such patents.

Specifications

• Reporter Gene: luc2A
• Storage Buffer: 10mM Tris HCl pH 7.4, 1mM EDTA
• Content And Storage: -30°C to -10°C
• Concentration: 1 μg/μL
• Primer: RV Primer 3 and RV Primer 4
• Quantity: 20 μg
• Vector: pGL4.10[luc2]
• For Use With (Application): Transcription regulation, virus-cell interactions, compound screening, post-translational modifications, GPCR signaling, cell signaling, promoter analysis