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Promega T3 RNA Polymerase

Catalog No. PRP4024 Shop All Promega Corporation Products
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Quantity:
1000 U
2500 U
Concentration:
10 to 20 U/μL
8 U/μL
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Catalog No. Quantity Concentration
PR-P4024 2500 U 8 U/μL
PR-P2083 1000 U 10 to 20 U/μL
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Catalog No. PR-P4024 Supplier Promega Supplier No. P4024
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Description

DNA-dependent RNA polymerase for in vitro transcription.

  • DNA-Dependent RNA Polymerase Specific for T3 Promoter Sequences
  • Exhibits extremely high affinity and specificity for T3 promoter sequences
  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (
  • Supplied at a concentration of 10-20u/μL (Cat.# P2083) and 80u/μL (Cat.# P4024)

Specifications

Concentration 8 U/μL
Color Colorless
Components 100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
Purity >90%
pH 7.7
For Use With (Application) Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Storage Buffer 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 2500 U
Product Type T3 RNA Polymerase
Form Liquid
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