Promega T4 DNA Ligase

Description

For ligation of DNA ends.

• Joins Two DNA Strands with Cohesive or Blunt Ends
• Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors)
• Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
• Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule

Specifications

• Concentration: 1 to 3 U/μL
• Color: Colorless
• Components: 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
• Purity: >90%
• pH: 7.4
• For Use With (Application): Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
• Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 500 U
• Product Type: T4 DNA Ligase
• Molecular Weight (g/mol): 68
• Form: Liquid