Promega T4 Polynucleotide Kinase Buffer Pack

Description

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

• Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides
• Can be inactivated by heating at 68°C for 10 minutes
• Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
• Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
• Supplied at a concentration of 5-10u/μL

Specifications

• Color: Colorless
• Components: 3 Tubes of T4 DNA Kinase 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25degC), 100mM MgCl2, 50mM DTT
• pH: 8
• For Use With (Application): For T4 DNA Kinase Enzyme
• Content And Storage: -30°C to -10°C
• Quantity: 3 x 500 μL
• Product Type: 4 DNA Kinase Buffer Pack
• Form: Liquid