Promega T4 RNA Ligase

Description

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

• Joins ssRNA or ssDNA onto the 5′-Phosphoryl End of ssRNA or ssDNA
• Adds [5′-32P] nucleoside 3′,5′-bis (phosphate) onto single-stranded RNA
• Can be inactivated by heating at 65°C for 15 minutes
• Supplied at a concentration of 10u/μL

Specifications

• Concentration: 10 U/μL
• Color: Colorless
• Components: 10X Reaction Buffer: 500mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 50mM DTT, 10mM ATP
• Purity: >90%
• pH: 7.5
• For Use With (Application): Ligation of single-stranded Nucleic Acids, 3' end-labeling of RNA, circularization of RNA and DNA Oligonucleotides, cloning full length cDNA
• Storage Buffer: 10mM Tris (pH 7.5 at 25°C), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1% Tween™20, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 500 U
• Product Type: T4 RNA Ligase
• Molecular Weight (g/mol): 43.5
• Form: Liquid