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Promega T7 RNA Polymerase
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Quantity:
10,000 U
1000 U
5000 U
Concentration:
10 to 20 U/μL
80 U/μL
Description
- DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
- >90% pure as determined by SDS polyacrylamide gel electrophoresis
- Free of detectable levels of contaminating RNase and DNase activity (
- Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates
Specifications
Specifications
Concentration | 10 to 20 U/μL |
Color | Colorless |
Components | 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl |
Purity | >90% |
pH | 7.7 |
For Use With (Application) | Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA |
Storage Buffer | 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol |
Content And Storage | -30°C to -10°C |
Source | Recombinant E. coli strain |
Quantity | 1000 U |
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