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Promega Terminal Deoxynucleotidyl Transferase

Catalog No. PRM1875 Shop All Promega Corporation Products
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1500 U
300 U
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PR-M1875 1500 U
PR-M1871 300 U
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Description

TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.

  • Adds Mononucleotides to the Terminal 3′-OH of ssDNA and Releases PPi
  • Adds mononucleotide tails to any type of 3′ end (3′ and 5′ overhangs or blunt ends) due to the presence of 1mM CoCl2 in the reaction buffer
  • Quality tested for labeling of apoptotic DNA ends using the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235 for each lot of enzyme
  • Supplied at a concentration of 30u/μL

Specifications

Concentration 30 U/μL
Color Colorless
Components 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH 6.4
For Use With (Application) Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Storage Buffer 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 1500 U
Product Type Terminal Deoxynucleotidyl Transferase
Form Liquid
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