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Invitrogen™ Propidium Iodide Ready Flow™ Reagent

Catalog No. R37169
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R37169 120 assays
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Catalog No. R37169 Supplier Invitrogen™ Supplier No. R37169
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Description

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays.

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 535/617 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh. or pipette
• Rapid staining of dead cells without wash steps

Propidium Iodide Ready Flow Reagent is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

Propidium iodide is membrane impermeant and therefore excluded from viable cells. It binds to DNA by intercalating between the bases, thereby binding both DNA and RNA. Propidium iodide is commonly excited with the 488 nm laser, but others such as the 532 and 561 nm lasers are also suited for PI excitation.

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Specifications

Cell Permeability Cell-Impermeant
Color Red
Content And Storage 2 dropper bottles, store (at room temperature)
Detection Method Fluorescence
Product Type Fluorescent Stain
Product Line Ready Flow
Quantity 120 assays
Reagent Type Nucleic Acid Stain
Sub Cellular Localization Nucleus
Excitation/Emission 535/617
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