Invitrogen™ Protocadherin Gamma Monoclonal Antibody (N159/5), PE

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545661

Catalog No. PIMA545661

Description

1 μg/mL of MA5-45661 was sufficient for detection of Protocadherin gamma (pan) in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins. This antibody was formerly sold as clone S159-5.

The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Specifications

• Antigen: Protocadherin Gamma
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: O60330, Q91XY4, Q9UN70, Q9UN71, Q9Y5F6, Q9Y5F7, Q9Y5F8, Q9Y5F9, Q9Y5G0, Q9Y5G1, Q9Y5G2, Q9Y5G3, Q9Y5G4, Q9Y5G5, Q9Y5G6, Q9Y5G7, Q9Y5G8, Q9Y5G9, Q9Y5H0, Q9Y5H1, Q9Y5H2, Q9Y5H3, Q9Y5H4
• Gene Symbols: Pcdhga1, Pcdhga10, Pcdhga11, PCDHGA12, Pcdhga2, PCDHGA3, PCDHGA4, Pcdhga5, PCDHGA6, Pcdhga7, PCDHGA8, Pcdhga9, PCDHGB1, PCDHGB2, PCDHGB3, Pcdhgb4, Pcdhgb5, Pcdhgb6, Pcdhgb7, Pcdhgc3, PCDHGC4, PCDHGC5
• Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: N159/5
• Conjugate: PE
• Gene: PCDHGA12
• Gene Alias: 3' RACE product; cadherin 20; cadherin 21; cadherin ME3; cadherin ME6; cadherin-20; Cadherin-21; cadherin-like 2; CDH20; CDH21; CDH-GAMMA-A5; FIB2; FIB3; fibroblast cadherin FIB2; fibroblast cadherin FIB3; fibroblast cadherin-2; Fibroblast cadherin-3; KIAA0327; KIAA0588; ME3; ME6; mKIAA0588; mKIAA4054; Pan-Gamma-Protocadherin-A; pc2c; PC43; PC-43; PCDH gamma A; Pcdh13; PCDH2; PCDHGA; PCDHGA1; PCDHGA10; PCDHGA11; PCDHGA12; Pcdhga2; PCDHGA3; Pcdhga4; PCDHGA5; PCDHGA6; PCDHGA7; Pcdhga8; PCDHGA9; PCDH-gamma-A; PCDH-GAMMA-A1; PCDH-gamma-A10; PCDH-GAMMA-A11; PCDH-gamma-A12; PCDH-GAMMA-A2; PCDH-gamma-A3; PCDH-gamma-A4; PCDH-GAMMA-A5; PCDH-gamma-A6; PCDH-GAMMA-A7; PCDH-gamma-A8; PCDH-gamma-A9; PCDH-GAMMA-B1; PCDH-GAMMA-B2; PCDH-gamma-B3; PCDH-GAMMA-B4; PCDH-GAMMA-B5; PCDH-GAMMA-B6; PCDH-gamma-B7; PCDH-GAMMA-C3; PCDH-gamma-C4; PCDH-gamma-C5; Pcdhgb1; PCDHGB2; PCDHGB3; PCDHGB4; PCDHGB5; Pcdhgb6; Pcdhgb7; PCDHGC3; Pcdhgc4; PCDHGC5; protocadherin 13; protocadherin 2; protocadherin 43; protocadherin gamma A12; protocadherin gamma B2; protocadherin gamma B6; protocadherin gamma C-VI; protocadherin gamma subfamily A, 1; protocadherin gamma subfamily A, 10; protocadherin gamma subfamily A, 11; protocadherin gamma subfamily A, 12; protocadherin gamma subfamily A, 2; protocadherin gamma subfamily A, 3; protocadherin gamma subfamily A, 4; protocadherin gamma subfamily A, 5; protocadherin gamma subfamily A, 6; protocadherin gamma subfamily A, 7; protocadherin gamma subfamily A, 8; protocadherin gamma subfamily A, 9; protocadherin gamma subfamily B, 1; protocadherin gamma subfamily B, 1, isoform 2; protocadherin gamma subfamily B, 2; protocadherin gamma subfamily B, 3; protocadherin gamma subfamily B, 4; protocadherin gamma subfamily B, 5; protocadherin gamma subfamily B, 6; protocadherin gamma subfamily B, 7; protocadherin gamma subfamily C, 3; protocadherin gamma subfamily C, 4; protocadherin gamma subfamily C, 5; protocadherin gamma-A1; Protocadherin gamma-A10; protocadherin gamma-A11; Protocadherin gamma-A12; protocadherin gamma-A2; Protocadherin gamma-A3; protocadherin gamma-A4; Protocadherin gamma-A5; protocadherin gamma-A6; Protocadherin gamma-A7; protocadherin gamma-A8; Protocadherin gamma-A9; protocadherin gamma-B1; Protocadherin gamma-B2; protocadherin gamma-B3; Protocadherin gamma-B4; protocadherin gamma-B5; Protocadherin gamma-B6; protocadherin gamma-B7; Protocadherin gamma-C3; protocadherin gamma-C4; Protocadherin gamma-C5; protocadherin-2; Protocadherin-43; UNQ371/PRO707
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 102552497, 102552705, 102552796, 116782, 252894, 252895, 252897, 26025, 291635, 291637, 364843, 364844, 498846, 498847, 498849, 498850, 5098, 553129, 56097, 56098, 56099, 56100, 56101, 56102, 56103, 56104, 56105, 56106, 56107, 56108, 56109, 56110, 56111, 56112, 56113, 56114, 641525, 641526, 680361, 680448, 8641, 93699, 93700, 93701, 93702, 93703, 93704, 93706, 93707, 93708, 93709, 93710, 93711, 93712, 93713, 93714, 93715, 93716, 93717, 93722, 93723, 93724, 9708
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov