Invitrogen™ PrP Polyclonal Antibody

Catalog No.	Quantity
PA1750	100 μL

Catalog No. PA1750 Supplier Invitrogen™ Supplier No. PA1750

Description

Rabbit Polyclonal Antibody

PA1-750 detects prion protein (PrP) from sheep tissues. PA1-750 has been successfully used in Western blot, immunoprecipitation and immunohistochemistry procedures. By Western blot, this antibody detects a 33-35 kDa protein representing PrPC and a 27-30 kDa protein representing PrPSc from sheep normal and infected brains. Immunohistochemical staining of PrP in formalin-fixed infected sheep brain with PA1-750 results in intense staining of PrP plaques which are known to exhibit an abnormally high concentration of PrP. PA1-750 immunizing peptide corresponds to amino acid residues 90-102 from hamster PrP. PA1-750 immunizing peptide (Cat. # PEP-034) is available for use in neutralization and control experiments.

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

Specifications

• Antigen: PrP
• Applications: Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: PRNP
• Gene Accession No.: P23907
• Gene Alias: AA960666; AI325101; Alternative prion protein; alternative prion protein; major prion protein; AltPrP; ASCR; BSE; CD230; CD230 antigen; CJD; GSS; KURU; major prion protein; major prion protein; alternative prion protein; major scrapie-associated fibril protein 1; MGC26679; p27-30; PK resistant core; prion protein; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); prion protein precursor; prion protein precursor PrP; prion protein PrP; prion protein variant a; prion protein variant b; prion protein, PrP; prion protein, structural; prion-related protein; PRIP; Prn; Prn-i; Prnp; Prn-p; PrP; PrP>C>; PrP27-30; PrP33-35C; PrPc; PrPSc; Sinc; SIP; TSE
• Gene Symbols: PRNP
• Host Species: Rabbit
• Immunogen: Synthetic Peptide: G(90) Q G G G T H N Q W N K P(102) G G C.
• Purification Method: Antigen Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 493887
• Target Species: Sheep
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG