Invitrogen™ PSME4 Polyclonal Antibody

Catalog No.	Quantity
PA11961	100 μL

Catalog No. PA11961 Supplier Invitrogen™ Supplier No. PA11961

Description

Rabbit Polyclonal Antibody

PA1-1961 detects PA200 from bovine, human, mouse and rat samples. PA1-1961 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing PA200 from bovine testis tissue extract. In mouse tissues, this antibody detects both a 200 kDa and a 160 kDa protein. In mouse tissues, the 200 kDa species is most abundant in testis whereas the 160 kDa species is enriched in the spleen. Immunofluorescence staining of PA200 in HeLa cells results in diffuse nuclear staining prior to ionizing radiation treatment. Immunofluorescence staining of PA200 in HeLa cells after ionizing radiation treatment results in focal aggregate staining within the nucleus.

Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. [UniProt].

Specifications

• Antigen: PSME4
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: Whole serum with 0.05% sodium azide
• Gene: PSME4
• Gene Accession No.: F1MKX4, Q14997, Q5SSW2
• Gene Alias: AA409398; AU041366; Kiaa0077; mKIAA0077; PA200; proteasome (prosome, macropain) activator subunit 4; proteasome activator 200 kDa; proteasome activator complex subunit 4; Proteasome activator PA200; proteasome activator subunit 4; Protein TEMO; Psme4; TEMO
• Gene Symbols: PSME4
• Host Species: Rabbit
• Immunogen: Synthetic peptide corresponding to residues TKLPKKRKRDPGSVG of human PA200 (aa 1734-1748).
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 103554, 23198, 498433, 528142
• Target Species: Human, Mouse, Rat, Bovine
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG