Invitrogen™ PST1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA534414

Catalog No. PIPA534414

Description

PA5-34414 can be used with blocking peptide PEP-1456.

Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain, which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.

Specifications

• Antigen: PST1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PST1
• Gene Alias: Cell wall mannoprotein PST1; D4214; Haze protective factor 2; HPF2; Protoplast secreted protein 1; PST1; Pst1p; YD9609.09; YDR055W
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 851625
• Content And Storage: Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: A6ZY20
• Gene Symbols: PST1
• Immunogen: A synthetic peptide from the amino terminus of yeast Pst1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Yeast
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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