PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP275766

Catalog No. NBP275766

Description

PU.1/Spi-1 Monoclonal specifically detects PU.1/Spi-1 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: PU.1/Spi-1
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot 0.5-1 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml
• Gene Accession No.: P17947
• Gene Symbols: SPI1
• Immunogen: Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )
• Purification Method: Protein A or G purified
• Research Discipline: Innate Immunity
• Gene ID (Entrez): 6688.0
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Immunohistochemistry (Paraffin)
• Clone: PU1/2146
• Conjugate: Unconjugated
• Formulation: 10 mM PBS with No Preservative
• Gene Alias: hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
• Host Species: Mouse
• Molecular Weight of Antigen: 40 kDa
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
• Content And Storage: Store at 4°C.
• Isotype: IgG2b κ