Invitrogen™ PUM1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530327

Catalog No. PIPA530327

Description

Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (97%), Chicken (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. It is a member of the PUF family comprised of evolutionarily conserved RNA-binding proteins. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), and 5'-UGUANAUA-3' that is related to the Nanos Response Element (NRE). PUM1 mediates post-transcriptonal repression of transcripts via different mechanisms - acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Mutations to the gene can result in spinocerebellar ataxia 47.

Specifications

• Antigen: PUM1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PUM1
• Gene Alias: AA517475; HsPUM; KIAA0099; mKIAA0099; PUM1; PUMH; PUMH1; pumilio 1; pumilio homolog 1; pumilio RNA binding family member 1; pumilio RNA-binding family member 1; pumilio-1; PUML1; Pumm
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 80912, 9698
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1.49 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q14671, Q80U78
• Gene Symbols: PUM1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 902 and 1186 of Human Pumilio 1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov