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Invitrogen™ PUMA alpha Polyclonal Antibody
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Catalog No. PIPA184091
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100 μg
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Catalog No. Quantity
PIPA184091 100 μg
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Catalog No. PIPA184091 Supplier Invitrogen™ Supplier No. PA184091
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Description

Rabbit Polyclonal Antibody

Predicted to react with mouse based on sequence homology. Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is human breast carcinoma. In Western blot, this antibody detects a band at ~23kDa in K562 cell lysate. Rabbit anti PUMA antibody recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2.

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
TRUSTED_SUSTAINABILITY

Specifications

Antigen PUMA alpha
Applications Immunohistochemistry (Paraffin), Western Blot
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.02% sodium azide
Gene BBC3
Gene Accession No. Q9BXH1
Gene Alias BBC3; BCL2 binding component 3; Bcl-2 binding component 3; bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; PUMA/JFY1
Gene Symbols BBC3
Host Species Rabbit
Immunogen Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha (aa 2-16).
Purification Method Affinity Chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 27113
Target Species Human
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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