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Invitrogen™ PUMA alpha Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA520007
Description
The PA5-20007 immunogen is located within the last 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA. In Western blot applications, this antibody detects a band at ~23kDa. A suggested positive control is K562 cell lysate. PA5-20007 can be used with blocking peptide PEP-0127. Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%).
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
Specifications
PUMA alpha | |
Polyclonal | |
Unconjugated | |
BBC3 | |
BBC3; BCL2 binding component 3; Bcl-2 binding component 3; bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; PUMA/JFY1 | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
170770, 27113 | |
Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C | |
Liquid |
Western Blot, Immunocytochemistry | |
1 mg/mL | |
PBS with 0.02% sodium azide | |
Q99ML1, Q9BXH1 | |
BBC3 | |
A 14 amino acid peptide near the carboxyl terminus human PUMAisoform 1. | |
100 μg | |
Primary | |
Human, Mouse | |
Antibody | |
IgG |
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