Invitrogen™ PUMA delta/gamma Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA141213

Catalog No. 01-675-772

Description

Some cross reactivity is seen with PUMA alpha. This sequence is 59% homologus to PUMA alpha. Suggested positive control: HeLa, NIH-3T3 cell lysate.

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

Specifications

• Antigen: PUMA delta/gamma
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BBC3
• Gene Alias: BBC3; BCL2 binding component 3; Bcl-2 binding component 3; bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; PUMA/JFY1
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 170770, 27113
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.05% BSA and 0.05% sodium azide
• Gene Accession No.: Q99ML1, Q9BXH1
• Gene Symbols: BBC3
• Immunogen: Synthetic peptide corresponding to residues 36-53 of PUMA-delta and 196-213 of PUMA-gamma.
• Quantity: 0.1 mg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG