Invitrogen™ PUMA alpha Monoclonal Antibody (10C5G1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515754

Catalog No. PIMA515754

Description

A suggested positive control is K562 cell lysate.

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

Specifications

• Antigen: PUMA alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q80ZG6, Q9BXH1
• Gene Symbols: BBC3
• Immunogen: Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: 10C5G1
• Conjugate: Unconjugated
• Gene: BBC3
• Gene Alias: BBC3; BCL2 binding component 3; Bcl-2 binding component 3; bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; PUMA/JFY1
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 27113, 317673
• Content And Storage: -20°C
• Form: Liquid