Invitrogen™ PUMA alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520007

Catalog No. PIPA520007

Description

The PA5-20007 immunogen is located within the last 50 amino acids of PUMA. The immunogenic sequence is identical between the A and B forms of PUMA. In Western blot applications, this antibody detects a band at ~23kDa. A suggested positive control is K562 cell lysate. PA5-20007 can be used with blocking peptide PEP-0127. Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%).

PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.

Specifications

• Antigen: PUMA alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BBC3
• Gene Alias: BBC3; BCL2 binding component 3; Bcl-2 binding component 3; bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; BH3-only; FLJ42994; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; PUMA/JFY1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 170770, 27113
• Content And Storage: Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q99ML1, Q9BXH1
• Gene Symbols: BBC3
• Immunogen: A 14 amino acid peptide near the carboxyl terminus human PUMAisoform 1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG