Pyruvate Kinase Polyclonal Antibody, Rockland™

Goat Polyclonal Antibody

Supplier:  Rockland Immunochemicals 20011780100

Catalog No. 50-199-0451

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-PYRUVATE KINASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle). This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

Specifications

• Antigen: Pyruvate Kinase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PKM
• Gene Alias: AA414905; AL024370; AL024424; CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; HEL-S-30; isozymes M1/M2; OIP3; OIP-3; Opa-interacting protein 3; p58; PK, muscle type; PK2; Pk-2; PK3; Pk-3; PKM; pkm.L; PKM12; PKM2; Pykm; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; pyruvate kinase M1/2 L homeolog; pyruvate kinase M1/2 L homeolog; pyruvate kinase, muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; TCB; THBP1; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; XELAEV_18018020mg
• Host Species: Goat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rabbit
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P11974
• Gene Symbols: PKM
• Immunogen: Pyruvate Kinase (Rabbit Muscle)
• Regulatory Status: RUO
• Gene ID (Entrez): 100008676
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized