Reproducibly quantify small RNA (⁓20 nucleotides or base pairs), even in the presence of contaminants such as salts, free nucleotides, solvents, detergents, and protein, with the Qubit microRNA Assay Kit. This assay is highly selective for small RNA over rRNA or large mRNA and can quantify small RNA in pure samples at levels as low as 0.5 ng in the assay tube. The assay detects all types of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA.Achieve easy and accurate quantification of microRNA (miRNA), even in the presence of contaminants such as salts, free nucleotides, solvents, detergents, and protein, with the Qubit microRNA Assay Kit. This assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Even in the presence of mRNA, miRNA levels as low as 0.5 ng can be detected (in pure samples) using the supplied protocol.
Features of the Qubit microRNA Assay Kit
• Accurately detects as little as 0.5 ng miRNA in the assay tube
• Core dynamic range of 1-100ng/mL and 5–500 ng/mL.
• Accurate results for sample concentrations ranging from 50 ng/mL to 100,000 ng/mL
The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations ranging from 50 ng/mL–100,000 ng/mL.
For Research Use Only. Not for use in diagnostic procedures.
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