R&D Systems™ Bovine Fibronectin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is 1-5 μg/cm2. Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives. Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 10.0-100 ng/mL.

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Specifications

• Formulation: Supplied as a 0.2μm filtered solution in Tris-HCl, NaCl and Urea.
• Gene ID (Entrez): 280794
• Name: Fibronectin
• Quantity: 5 mg
• Source: Bovine plasma-derived Fibronectin protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. Avoid vortexing and excessive agitation.
• Conjugate: Unconjugated
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.