R&D Systems™ Fraction II Degradation Kit, HeLa Cells

Description

For the controlled degradation of exogenously added or endogenously contained substrate proteins through the ubiquitin proteasome pathway (UPP)

Fraction II is the protein fraction of a cell extract that binds to anion exchange resin: it contains E1, most E2s, some E3s, UCHs, and the proteasome (20S and 26S) and is essentially free of ubiquitin and ATP

Protein substrate of choice can be added exogenously or if the protein of interest is already contained in the HeLa fraction, its degradation may be tracked via immuno-detection
Tracking degradation of the target protein depends on the detection method and users preference (typically SDS-PAGE)

Specifications

• Description: Fraction II Degradation Kit, HeLa Cells
• For Use With (Application): Controlled degradation of exogenously added or endogenously contained substrate proteins through the ubiquitin proteasome pathway (UPP)
• Quantity: 1 Kit