R&D Systems™ Recombinant Human B7-H3 (4Ig)/B7-H3b Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and/or 10 μg/mL rhB7-H3 (4Ig)/B7-H3b. The presence of rhB7-H3 (4Ig)/B7-H3b at 10 μg/mL inhibited the anti-CD3 response by >30%. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: Q5ZPR3
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 80381
• Molecular Weight (g/mol): M.W. (Observed): 78-90 kDa, reducing conditions; M.W. (theoretical): 48 kDa
• Name: B7-H3 (4Ig)/B7-H3b
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H3 protein Gly27-Thr461, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD276
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: B7-H3/CD276