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R&D Systems™ Human CD3+ T Cell Enrichment Column, Mini
Description
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents
Total CD3+ cell recovery from CD3+ cells loaded ranges between 32% and 40%
Purity of recovered cells ranges between 85% and 95%
These enriched T cell populations are then available for tissue culture, activation studies, tissue typing, immune status monitoring, and flow cytometry
Specifications
Specifications
| Content And Storage | Store the unopened product at 2°C to 8°C. Do not use past expiration date. |
| For Use With (Application) | Designed to prepare purified human T cell populations via high affinity negative selection |
| Includes | Human T Cell Selection Columns; 10X Column Buffer Concentrate |
| Quantity | 25/Pk. |
| Type | Human CD3+ T Cell Enrichment Column |
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