R&D Systems™ Human CD3+ T Cell Enrichment Column, Mini

Description

Designed to prepare purified human T cell populations via high affinity negative selection

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents
Total CD3+ cell recovery from CD3+ cells loaded ranges between 32% and 40%
Purity of recovered cells ranges between 85% and 95%
These enriched T cell populations are then available for tissue culture, activation studies, tissue typing, immune status monitoring, and flow cytometry

Specifications

• Content And Storage: Store the unopened product at 2°C to 8°C. Do not use past expiration date.
• For Use With (Application): Designed to prepare purified human T cell populations via high affinity negative selection
• Includes: Human T Cell Selection Columns; 10X Column Buffer Concentrate
• Quantity: 25/Pk.
• Type: Human CD3+ T Cell Enrichment Column