R&D Systems Human CNTF Recombinant Protein
Manufacturer: R&D Systems 257NT050/CF
DescriptionMeasured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED 50 for this effect is 0.03-0.18 µg/mL. The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR ) . Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED 50 for this effect is 1-3 ng/mL.
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|Lyophilized from a 0.2μm filtered solution in PBS, NaCl, EDTA, DTT and CHAPS.|
|97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2 - 22.8 kDa.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, 2 to 8° C under sterile conditions after reconstitution.|
|<0.1 EU per 1μg of the protein by the LAL method.|
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