R&D Systems™ Recombinant Human Nectin-2/CD112 Protein

Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to bind with Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN ) in a functional ELISA.

Specifications

• Accession Number: NP_002847
• For Use With (Application): Binding Activity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 5819
• Molecular Weight (g/mol): M.W. (Observed): 43-50 kDa, reducing conditions; M.W. (theoretical): 36.3 kDa
• Name: Nectin-2/CD112
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Nectin-2/CD112 protein Gln32-Leu360, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening.
• Structural Form: Monomer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: NECTIN2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 50 μg/mL The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Protein: Nectin-2/CD112