R&D Systems™ Recombinant Human PDGF-BB Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
• Gene ID (Entrez): 5155
• Molecular Weight (g/mol): 12.3 kDa (monomer)
• Name: PDGF-BB
• Quantity: 10 μg
• Source: E. coli-derived human PDGF-BB protein Ser82-Thr190
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.