R&D Systems™ Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP-1/PCP (Catalog # 1927-ZN ). >50% of full-length Pro-Collagen I alpha 1 is cleaved by Recombinant Human BMP-1/PCP (Catalog # 1927-ZN ), as measured under the described conditions.

Specifications

• Accession Number: P02452
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 1277
• Molecular Weight (g/mol): M.W. (Observed): 79-83 kDa, 54-56 kDa, 35-36 kDa, reducing conditions; M.W. (theoretical): 65 kDa (full-length), 40 kDa, 28 kDa
• Name: Pro Collagen I alpha 1
• Quantity: 20 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Collagen I alpha 1 protein Gln23-Lys277 + Gly1094-Leu1464, with an N-terminal 6-His tag.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: COL1A1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Collagen I alpha 1