R&D Systems™ Recombinant Human SIRP alpha/CD172a Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Subramanian, S. et al. (2006) Blood 107:2548. Red blood cells, added at 10 x 106 cells/mL (100 μL/well) to SIRP alpha coated plates, will adhere after 1 hour at 37° C. The ED50 for this effect is 0.6-2.4 μg/mL. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: NP_542970
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 140885
• Molecular Weight (g/mol): M.W. (Observed): 70-105 kDa, reducing conditions; M.W. (theoretical): 64 kDa (monomer)
• Name: SIRP alpha/CD172a Fc Chimera
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Human SIRP alpha (Gly27-Arg370) & (Glu31-Arg370) (N-terminus) Accession # NP_542970 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: SIRPA
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: SIRP alpha/CD172a