R&D Systems™ Recombinant Mouse LIGHT/TNFSF14 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142. The ED50 for this effect is 2-5 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ) and recombinant human IFN-gamma .

Specifications

• Accession Number: Q9QYH9
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 8740
• Molecular Weight (g/mol): M.W. (Observed): 24-34 kDa, reducing conditions; M.W. (theoretical): 21 kDa
• Name: LIGHT/TNFSF14
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived mouse LIGHT/TNFSF14 protein MHHHHHHHHHH GGGSGGGSGGGS IEGR Mouse LIGHT (Asp72-Val239) (N-terminus) Accession # Q9QYH9 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Tnfsf14
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: LIGHT/TNFSF14