R&D Systems™ Recombinant Mouse Plasma Kallikrein/KLKB1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The specific activity is >300 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: NP_032481
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Gene ID (Entrez): 3818
• Molecular Weight (g/mol): M.W. (Observed): 80 kDa, reducing conditions; M.W. (theoretical): 70 kDa
• Name: Plasma Kallikrein/KLKB1
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Plasma Kallikrein/KLKB1 protein Gly20-Ala638, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Pro form
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Klkb1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in sterile 25 mM MES and 150 mM NaCl, pH 5.5.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: Plasma Kallikrein/KLKB1