R&D Systems Recombinant Human GDF?9

Growth Differentiation Factor 9

Manufacturer: R&D Systems 8266G9010/CF

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Catalog No. 82-66G-9010CF

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    Accession Number O60383
    Conjugate Unlabeled
    Formulation Lyophilized from a 0.2μm filtered solution in HCl.
    Molecular Weight 16 kDa (monomer)
    Name GDF-9
    Purity >95%, by SDS-PAGE with silver staining.
    Quantity 10μg
    Recombinant Yes
    Source Human

    Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

    N-terminal Sequence Analysis: Gly320
    SDS-PAGE: 20-22 kDa, reducing conditions
    Activity: Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 50-100ng/mL of Recombinant Human GDF-9 can effectively induce Smad2 phosphorylation.
    Reconstitution: Reconstitute at 100µg/mL in strerile 4mM HCl.

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