R&D Systems Rhesus Macaque IL-18/IL-1F4 Recombinant Protein
Manufacturer: R&D Systems 2548RM025/CF
DescriptionMeasured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha . The ED 50 for this effect is 1-4 ng/mL in the presence of 20 ng/mL recombinant human TNF-alpha .
|Supplied as a 0.2μm filtered solution in MOPS, Na2SO4, EDTA and DTT.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after opening.|
|97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.|
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