R&D Systems™ Recombinant Yeast GST-Ubiquitin Activating Enzyme (UBE1)

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Recombinant Yeast GST-Ubiquitin E1 Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial GST-Ubiquitin E1 Enzyme (UBE1) concentration of 50-200 nM.

Order Info

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately

Specifications

• Accession Number: P22515
• For Use With (Application): Bioactivity
• Formulation: 0.71 mg/mL (5 μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Gene ID (Entrez): 7317
• Molecular Weight (g/mol): M.W. (theoretical): 141 kDa
• Name: GST Ubiquitin-activating Enzyme/UBE1, Carrier Free
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived yeast Ubiquitin-activating Enzyme/UBE1 protein Contains an N-terminal GST (glutathione S-transferase) tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: UBA1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: Ubiquitin-activating Enzyme/UBE1