Invitrogen™ RAB3D Monoclonal Antibody (5E9), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545969

Catalog No. PIMA545969

Description

A 1:1,000 dilution of MA5-45969 was sufficient for detection of RAB3D in 10 μg of A549, T-47D cells, mouse and rat brain, kidney by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Detects approximately 24 kDa. Does not show cross reactivity to RAB3A, RAB3B, or RAB3C.

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.

Specifications

• Antigen: RAB3D
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: O95716, P35276, Q63942
• Gene Symbols: Rab3d
• Immunogen: Full length recombinant RAB3D protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: 5E9
• Conjugate: APC
• Gene: Rab3d
• Gene Alias: C130057E11Rik; D2-2; glioblastoma overexpressed; GOV; GTP-binding protein Rab-3D; RAB16; Rab3d; Rab3D upregulated with myeloid differentiation; RAB3D, member RAS oncogene family; RAD3D; Ras-related protein Rab-3D
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 140665, 19340, 9545
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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