AKT1 (phospho S473), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.

Supplier:  Abnova Corporation PAB25274

Catalog No. 89-126-939

Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq

Sequence: Q-F-Sp-Y-S

Specifications

• Antigen: AKT1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
• Gene: AKT1
• Gene Alias: AKT/MGC99656/PKB/PKB-ALPHA/PRKBA/RAC/RAC-ALPHA
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 207
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

• Applications: Immunohistochemistry (PFA fixed), Western Blot
• Concentration: 1 mg/mL
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
• Formulation: In PBS (without mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Accession No.: P31749
• Gene Symbols: AKT1
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid

For Research Use Only