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MilliporeSigma™ Rabbit anti-BIG2, Polyclonal,
Rabbit Polyclonal Antibody
Supplier: MilliporeSigma™ ABS991
Description
Specifically detects BIG2 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting
Mammalian BIG2 (BFA-inhibited guanine nucleotide-exchange protein 2) is encoded by the Arfgef2 gene in rat (NCBI Gene ID 296380). The fungal antibiotic Brefeldin A (BFA) causes disassembly of the Golgi complex and ER swelling in mammalian cells by inhibiting three ADP-ribosylation factor (ARF) guanine nucleotide-exchange factors (GEFs), BIG1, BIG2, and GBF1, all of which interact with various ARFs via their respective Sec7 domains. ARF4/5 activation by GBF1 is shown to facilitate BIG1/2 recruitment to the trans-Golgi network (TGN) and endosomes, where BIG1/2 mediate ARF1/3 activation and lead to recruitment of clathrin adaptors. Independent of its Sec7 domain-mediated GEF activity, BIG2 is also known to interact with other cellular proteins and function as a scaffold for assembling multimolecular complexes involved in several cellular processes (PMID 23386609, 23918382, and 25097827). Anti-BIG2, is a rabbit antiserum raised against rat BIG2 C-terminal sequence (UniProt Q7TSU1) that is not present in BIG1. Antiserum cross-reactivity toward human (UniProt Q9Y6D5) and murine (UniProt A2A5R2) BIG2 has been confirmed.
Specifications
BIG2 | |
Polyclonal | |
Q7TSU1 | |
Rabbit | |
Unpurified | |
RUO | |
Primary | |
Human, Mouse, Rat | |
Serum |
Immunocytochemistry, Western Blot | |
Rabbit polyclonal serum with 0.05% sodium azide. | |
Arfgef2; Arfgep2; Big2 | |
KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat BIG2. | |
100 μL | |
Signaling | |
NP_851597 | |
Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. |
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