Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. ABS991MI

MilliporeSigma™ Rabbit anti-BIG2, Polyclonal,

Catalog No. ABS991MI Shop All MilliporeSigma Products
Change view
Click to view available options
Quantity:
100 μL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
ABS991MI 100 μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. ABS991MI Supplier MilliporeSigma™ Supplier No. ABS991
Only null left
Add to Cart
Add to Cart

Description

Rabbit Polyclonal Antibody

Specifically detects BIG2 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting

Mammalian BIG2 (BFA-inhibited guanine nucleotide-exchange protein 2) is encoded by the Arfgef2 gene in rat (NCBI Gene ID 296380). The fungal antibiotic Brefeldin A (BFA) causes disassembly of the Golgi complex and ER swelling in mammalian cells by inhibiting three ADP-ribosylation factor (ARF) guanine nucleotide-exchange factors (GEFs), BIG1, BIG2, and GBF1, all of which interact with various ARFs via their respective Sec7 domains. ARF4/5 activation by GBF1 is shown to facilitate BIG1/2 recruitment to the trans-Golgi network (TGN) and endosomes, where BIG1/2 mediate ARF1/3 activation and lead to recruitment of clathrin adaptors. Independent of its Sec7 domain-mediated GEF activity, BIG2 is also known to interact with other cellular proteins and function as a scaffold for assembling multimolecular complexes involved in several cellular processes (PMID 23386609, 23918382, and 25097827). Anti-BIG2, is a rabbit antiserum raised against rat BIG2 C-terminal sequence (UniProt Q7TSU1) that is not present in BIG1. Antiserum cross-reactivity toward human (UniProt Q9Y6D5) and murine (UniProt A2A5R2) BIG2 has been confirmed.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BIG2
Applications Immunocytochemistry, Western Blot
Classification Polyclonal
Formulation Rabbit polyclonal serum with 0.05% sodium azide.
Gene Accession No. Q7TSU1
Gene Symbols Arfgef2; Arfgep2; Big2
Host Species Rabbit
Immunogen KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat BIG2.
Purification Method Unpurified
Quantity 100 μL
Regulatory Status RUO
Research Discipline Signaling
Primary or Secondary Primary
Gene ID (Entrez) NP_851597
Target Species Human, Mouse, Rat
Content And Storage Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
Form Serum
Show More Show Less

For Research Use Only

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.