MilliporeSigma™ Rabbit anti-BIG2, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS991

Catalog No. ABS991MI

Description

Specifically detects BIG2 in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting

Mammalian BIG2 (BFA-inhibited guanine nucleotide-exchange protein 2) is encoded by the Arfgef2 gene in rat (NCBI Gene ID 296380). The fungal antibiotic Brefeldin A (BFA) causes disassembly of the Golgi complex and ER swelling in mammalian cells by inhibiting three ADP-ribosylation factor (ARF) guanine nucleotide-exchange factors (GEFs), BIG1, BIG2, and GBF1, all of which interact with various ARFs via their respective Sec7 domains. ARF4/5 activation by GBF1 is shown to facilitate BIG1/2 recruitment to the trans-Golgi network (TGN) and endosomes, where BIG1/2 mediate ARF1/3 activation and lead to recruitment of clathrin adaptors. Independent of its Sec7 domain-mediated GEF activity, BIG2 is also known to interact with other cellular proteins and function as a scaffold for assembling multimolecular complexes involved in several cellular processes (PMID 23386609, 23918382, and 25097827). Anti-BIG2, is a rabbit antiserum raised against rat BIG2 C-terminal sequence (UniProt Q7TSU1) that is not present in BIG1. Antiserum cross-reactivity toward human (UniProt Q9Y6D5) and murine (UniProt A2A5R2) BIG2 has been confirmed.

Specifications

• Antigen: BIG2
• Classification: Polyclonal
• Gene Accession No.: Q7TSU1
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Serum

• Applications: Immunocytochemistry, Western Blot
• Formulation: Rabbit polyclonal serum with 0.05% sodium azide.
• Gene Symbols: Arfgef2; Arfgep2; Big2
• Immunogen: KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat BIG2.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NP_851597
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only