MilliporeSigma™ Rabbit anti-Bora, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE1858

Catalog No. ABE1858

Description

Specifically detects Bora in Human samples, and it is validated for Immunoprecipitation, Western Blotting

Protein aurora borealis (UniProt Q6PGQ7; also known as HsBora) is encoded by the BORA (also known as C13orf34) gene (Gene ID 79866) in human. Originally identified as a co-factor for Aurora A-dependent asymmetrical cell divisions, Bora is an adaptor/scaffold protein that plays an important role in mitosis by mediating Aurora A-dependent Plk1 activation, and Bora depletion abolishes both Plk1 and Cdk1 activation. Cellular Bora activity is negarively regulated by Plk1- and Cdk1-mediated phosphorylation. Cdk1 initially phosphorylates Bora on S252, which primes Bora for Plk1-catalyzed S497 and T501 phosphorylation, leading to SCFβTrCP-mediated Bora ubiquitination and subsequent proteasomal degradation. The negative feed-back regulation of Bora by the kinase it activates is important, as uncontrolled Bora overexpression perturbs Aurora A and Plk1 localization, leading to lengthened metaphase. Similar to the counter regulation between Bora and Cdk1/Plk1 in somatic cells, Bora degradation is reported to be regulated via phosphorylation by Cdk1 and Plx1 in xenopus ooctyes. Cdk1 is reported to phosphorylate Bora on Thr52, which blocks its degradation. Fertilization induced calcium surge jump-starts the oscillations of the embryonic cycles by inducing Calcineurin-catalyzed Bora pThr52 dephosphorylation, leading to Bora degradation.

Specifications

• Antigen: Bora
• Classification: Polyclonal
• Gene Accession No.: Q6PGQ7
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Serum

• Applications: Immunoprecipitation, Western Blot
• Formulation: Rabbit polyclonal antibody in serum with 0.05% sodium azide.
• Gene Symbols: BORA; C13orf34
• Immunogen: GST-tagged recombinant human Bora N-terminal fragment.
• Quantity: 100 μL
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_079084
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only