CDC27 (phospho T244), Rabbit, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27.

Supplier:  Abnova Corporation PAB9988

Catalog No. 89-134-552

Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Specifications

• Antigen: CDC27
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27.
• Formulation: In 20mM KH₂PO₄, 150mM NaCl, pH 7.2 (0.01% sodium azide)
• Gene Accession No.: P30260;NP_001247
• Gene Symbols: CDC27
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T244 of human CDC27.
• Regulatory Status: RUO
• Gene ID (Entrez): 996
• Target Species: Bovine, Canine, Chicken, Chimpanzee, Human, Mouse, Rat
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Dilution: ELISA (1:8000-1:30000) Western Blot (1:300-1:2000) The optimal working dilution should be determined by the end user.
• Gene: CDC27
• Gene Alias: APC3/CDC27Hs/D0S1430E/D17S978E/HNUC
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Reactivity occurs against human CDC27 pT244 protein and This antibody is specific to the phosphorylated form of the protein. This antibody does not cross-react with CDC27 phosphorylated at other sites.
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

For Research Use Only