CHEK2 (phospho S33), Rabbit anti-Human, Polyclonal Antibody, Abnova™

Catalog No.	Quantity
89-127-538	100 μg

Catalog No. 89-127-538 Supplier Abnova Corporation Supplier No. PAB25908

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK2.

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Sequence: S-S-Sp-Q-S

Specifications

• Antigen: CHEK2
• Applications: Immunohistochemistry (PFA fixed)
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK2.
• Dilution: Immunohistochemistry (1:50-1:100) The optimal working dilution should be determined by the end user.
• Formulation: In PBS (without mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene: CHEK2
• Gene Accession No.: O96017
• Gene Alias: CDS1/CHK2/HuCds1/LFS2/PP1425/RAD53
• Gene Symbols: CHEK2
• Host Species: Rabbit
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S33 of human CHEK2.
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11200
• Target Species: Human
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid

For Research Use Only