COL1A1, Rabbit, Polyclonal Antibody, Abnova™

Catalog No.	Quantity
89-118-877	100 μL

Catalog No. 89-118-877 Supplier Abnova Corporation Supplier No. PAB17205

Description

Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish

Specifications

• Antigen: COL1A1
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Description: Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.
• Dilution: Western Blot (1:1000) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user.
• Formulation: In 10mM HEPES, 150mM NaCl, pH 7.5 (50% glycerol, 0.1mg/mL BSA)
• Gene: COL1A1
• Gene Accession No.: P02452
• Gene Alias: OI4
• Gene Symbols: COL1A1
• Host Species: Rabbit
• Immunogen: A synthetic peptide corresponding to amino acids at C-terminus of human COL1A1.
• Purification Method: Affinity purification
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1277
• Test Specificity: This antibody has been directly tested for reactivity in human, mouse, rat and sheep. Based on the homology of the immunogen this antibody is expected to recognize the collagen I 1 polypeptide in all mammals, birds, and amphibians. specific to the ∼ 140 KDa telopeptide portion of the collagen I 1 polypeptide. This antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
• Target Species: Avian, Human, Mammalia, Mouse, Rat
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid

For Research Use Only