DFFA Rabbit anti-Human, Polyclonal Antibody, Abnova
Rabbit polyclonal antibody raised against synthetic peptide of DFFA.
Manufacturer: Abnova Corporation PAB13068
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFFB) and 45kDa (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
|Western Blot (1:1000-1:2000) Immunoprecipitation (1:1000-1:2000) The optimal working dilution should be determined by the end user.|
|A synthetic peptide corresponding to amino acids 313-331 of human DFFA.|
|Immunocytochemistry, Western Blot|
|Rabbit polyclonal antibody raised against synthetic peptide of DFFA.|
|In PBS (0.02% sodium azide)|
|Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
For Research Use Only