E2F1 (phospho S364), Rabbit, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1.

Supplier:  Abnova Corporation PAB10004

Catalog No. 89-114-545

Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq

Specifications

• Antigen: E2F1
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of E2F1.
• Formulation: In 20mM KH₂PO₄, 150mM NaCl, pH 7.2 (0.01% sodium azide)
• Gene Accession No.: Q01094;NP_005216
• Gene Symbols: E2F1
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S364 of human E2F1.
• Regulatory Status: RUO
• Gene ID (Entrez): 1869
• Target Species: Chimpanzee, Human
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (PFA fixed), Western Blot
• Conjugate: Unconjugated
• Dilution: ELISA (1:20000-1:100000) Western Blot 1:250-1:2000 Immunohistochemistry (2-20 ug/mL) The optimal working dilution should be determined by the end user.
• Gene: E2F1
• Gene Alias: E2F-1/RBAP1/RBBP3/RBP3
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Reactivity occurs against human E2F-1 pS364 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. This antibody does not cross-react with E2F-1 phosphorylated at other sites.
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

For Research Use Only